Journal: Cell host & microbe
Article Title: Amino acid competition shapes Acinetobacter baumannii gut carriage
doi: 10.1016/j.chom.2025.07.003
Figure Lengend Snippet: (A) α-Diversity was calculated from 16S rRNA gene sequencing at 0 and 9 DPI of A. baumannii WT and Δ astO ( n = 10; data combined from mice in and ; mean ± SD are shown; p by one-way ANOVA with Sidak’s multiple comparisons). (B) Representative image of A. baumannii WT colonization at 10 DPI in the colon, visualized by MiPACT-HCR. Scale bar is 100 μm. Red, pan-bacteria HCR; blue, DAPI; magenta, WGA-lectin; green, anti- Acinetobacter HCR. From mice in and . (C) Experimental design with germ-free mice. (D) A. baumannii CFU from fecal samples of germ-free mice ( n = 6 female and n = 4 male mice, p by two-way ANOVA with Sidak’s multiple comparisons; experiment was repeated three times with similar results). (E) L-ornithine was quantified in chow and fecal samples (chow samples are n = 3 [LOD = 1 nmol/g], fecal samples are n = 10 mice shown in , , and [LOD = 5 nmol/g], mean ± SD, p by two-way ANOVA with Fisher’s least significant difference multiple comparisons only on fecal samples). (F) Principal-component analysis of amino acids in fecal samples at 0 and 10 DPI with chow samples shown in each plot ( n = 10 mice shown in , , and , chow n = 3). Lines connect CFU of both strains enumerated from the same mouse. DPI, days post inoculation; MiPACT-HCR, microbial identification after passive CLARITY technique via hybridization chain reaction; WGA, wheat germ agglutinin; CFU, colony-forming units; LOD, approximate limit of detection; PC, principal component. See also and .
Article Snippet: Acinetobacter gyllenbergii , DSMZ , LP698.
Techniques: Sequencing, Bacteria, Hybridization